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Profile: Mingfu Wu

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Mingfu Wu, Ph.D.
Associate Professor of Pharmacology

Health 2
University of Houston College of Pharmacy
4849 Calhoun, Room 5014
Houston, TX 77204-5039

mwu25@central.uh.edu
713-743-9880

Education & Post-graduate Training

Postoc 2007-2010, UT Southwestern Medical Center, Dallas, Texas

Postdoc 2006-2007, Duke University, Durham, North Carolina

Ph.D. in Biology, 2005, Kansas State University, Manhattan, Kansas

Research Interests

1. My lab aims to determine the etiology and then the treatment for the left ventricular non-compaction cardiomyopathy.

2. Another project will determine how epicardial development regulates cardiac morphogenesis and explore methods to treat congenital heart defects caused by abnormal epicardial development.

3. We are also screening chemicals that promote cardiomyocyte maturation during cell-reprogramming and cardiomyocyte proliferation during cardiac regeneration.

Selected Publications

Bin Zhou; Yan Li; Zan Lv; Shaohua Zhang; Zhuo Wang; Lingjuan He; Muxue Tang; Wenjuan Pu; Huan Zhao; Zhenqian Zhang; Qihui Shi; Dongqing Cai; Mingfu Wu; Guohong Hu; Kathy O. Lui; Jing Feng; Maria Angela Nieto, Bin Zhou. Genetic Fate Mapping of Transient Cell Fate Reveals N-cadherin Activity and Function in Tumor Metastasis. Dev Cell 2020 (Accepted)

Xue B, Liu YX, Dong B, Wingfield JL, Wu M, Sun J, Lechtreck KF, Fan ZC. Intraflagellar transport protein RABL5/IFT22 recruits the BBSome to the basal body through the GTPase ARL6/BBS3. Proc Natl Acad Sci U S A.2020 Feb 4;117(5):2496-2505. doi: 10.1073/pnas.1901665117. Epub 2020 Jan 17. PubMed PMID: 31953262; PubMed Central PMCID: PMC7007519.

Choi M, Lu YW, Zhao J, Wu M, Zhang W, Long X. Transcriptional control of a novel long noncoding RNA Mymsl in smooth muscle cells by a single Cis-element and its initial functional characterization in vessels. J Mol Cell Cardiol. 2020 Jan;138:147-157. doi: 10.1016/j.yjmcc.2019.11.148. Epub 2019 Nov 18. PubMed PMID: 31751568; PubMed Central PMCID: PMC7036038.

Lianjie Miao&, Jingjing Li&, Jian Li, Yangyang Lu, David Shieh, Joseph E. Mazurkiewicz, Margarida Barroso, John J. Schwarz, Hongbo Xin, Harold A. Singer, Peter Vincent, Weimin Zhong, Glenn L. Radice, Leo Q Wan, Zhen-Chuan Fan, Guoying Huang, and Mingfu Wu*. Cardiomyocyte orientation modulated by the Numb Family Proteins-N-Cadherin axis is essential for ventricular wall morphogenesis. Proc Natl Acad Sci U S A. 2019 Jul 30;116(31):15560-15569. doi: 10.1073/pnas.1904684116. Epub 2019 Jul 12. PubMed PMID: 31300538.

Ray P, Chin AS, Worley KE, Fan J, Kaur G, Wu M, Wan LQ. Intrinsic cellular chirality regulates left-right symmetry breaking during cardiac looping. Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):E11568-E11577. doi: 10.1073/pnas.1808052115. Epub 2018 Nov 20. PubMed PMID: 30459275; PubMed Central PMCID: PMC6294912.

Wu M. Mechanisms of Trabecular Formation and Specification During Cardiogenesis. Pediatric Cardiology, 2018, 2018 Mar 28. doi: 10.1007/s00246-018-1868-x (Invited Review).

Lianjie Miao, Jingjing Li, Jun Li, Xueying Tian, Yangyang Lu, Saiyang Hu, David Shieh, Ryan Kanai, Bo-yang Zhou, Bin Zhou, Jiandong Liu, Anthony B. Firulli, James F. Martin, Harold Singer, Bin Zhou, Hongbo Xin* & Mingfu Wu* Notch signaling regulates Hey2 expression in a spatiotemporal dependent manner during cardiac morphogenesis and trabecular specification. Scientific Reports. 2018 Feb 8;8(1):2678. doi: 10.1038/s41598-018-20917-w.

Dong B, Wu S, Wang J, Liu YX, Peng Z, Meng DM, Huang K, Wu M, Fan ZC. Chlamydomonas IFT25 is dispensable for flagellar assembly but required to export the BBSome from flagella. Biol Open (2017) doi: 10.1242/bio.026278

#Jingjing Li, #Lianjie Miao, Chen Zhao, Wasay Mohiuddin Shaikh Qureshi, David Shieh, Hua Guo, Yangyang Lu, Saiyang Hu, Alice Huang, Lu Zhang, Chen-leng Cai, Leo Q Wan, Peter Vincent, Harold A. Singer, Yi Zheng, Ondine Cleaver, Zhen-Chuan Fan and Mingfu Wu*. CDC42 is required for epicardial and pro-epicardial development by mediating FGF receptor trafficking to plasma membrane. Development (2017) 144, 1635-1647 doi:10.1242/dev.147173 This paper was featured: CDC42 shapes up the epicardium http://dev.biologists.org/content/144/9/e0902

Jinjing Zhao*, Wen Wu*, Wei Zhang, Emiley Tou, Jiemei Ye, Ping Gao, David Jourd'heuil, Harold A. Singer,Mingfu Wu and Xiaochun Long. TSPAN2 is a novel transmembrane protein closely associated with vascular smooth muscle contractile phenotype and is regulated by the TGFβ1/SMAD and MYOCD/SRF pathways. FESB J. 2017. Jun;31(6):2576-2591.

Wasay-Mohiuddin Shaikh-Qureshi, David Shieh, Lianjie Miao, Jingjing Li, Mingfu Wu*. Imaging cleared embryonic heart and postnatal heart at single cell resolution. J Vis Exp. 2016 Oct 7;(116).

Li J, Miao L, Shieh D, Spiotto E, Li J, Zhou B, Paul A, Schwartz RJ, Firulli AB, Singer HA, Huang G, Wu M*. Single-Cell Lineage Tracing Reveals that Oriented Cell Division Contributes to Trabecular Morphogenesis and Regional Specification. Cell Rep. 2016 Apr 5;15(1):158-170. doi: 10.1016/j.celrep.2016.03.012. Epub 2016 Mar 24. PubMed PMID: 27052172; PubMed Central PMCID: PMC4826812.

The papers above are introduced by The Scientist: http://www.the-scientist.com/?articles.view/articleNo/47495/title/How-to-Track-Cell-Lineages-As-They-Develop/

Wu M* and Li, J. Numb family proteins: novel players in cardiac morphogenesis and cardiac progenitor cell differentiation. Biomol Concepts. April 2015. Invited Review.

Wu M* and Meng, F. Has the cardiac stem cell controversy settled down? Sci China Life Sci. June 20 (2014).

Zhao C, Guo H, Li J, Myint T, Pittman W, Yang L, Zhong W, Schwartz RJ, Schwarz JJ, Singer HA, Tallquist MD, and Wu M*. Numb family proteins are essential for cardiac morphogenesis and progenitor differentiation. Development. 2014 Jan;141(2):281-95.

Wang Y, Hu G, Liu F, Wang X, Wu M, Schwarz JJ, Zhou J. Deletion of YAP Specifically in Cardiac and Vascular Smooth Muscle Cells Reveals a Crucial Role for YAP in Mouse Cardiovascular Development. Circ Res. 2014 Jan 29

Wu M, Christopher L. Smith, James A. Hall, Ivy Lee, Kate luby-Phelps, and M. Tallquist. Epicardial spindle orientation controls cell entry into the myocardium. Developmental Cell, 19(1):114-25 (2010).

M. Wu, H. Kwon, F. Rattis, J. Blum, C. Zhao, R. Ashkenazi, T. L. Jackson, N. Gaiano, T.Oliver and T. Reya. Imaging hematopoietic precursor division in real time. Cell Stem Cell. 1: 541-554 (2007).

Presentations

“Mechanism of Trabeculation.” AHA Scientific Sessions, Philadelphia, PA, Nov. 2019. (Invited Speaker).

“Cardiomyocyte orientation modulated by the Numb Family Proteins-N-Cadherin axis is essential for ventricular wall morphogenesis,” AHA Basic Cardiovascular Sciences 2018 Scientific Sessions (July 30-Aug. 2), San Antonio, TX, Aug. 1, 2018. (Platform Oral Presentation)

“Numb modulates cardiac morphogenesis by regulating N-Cadherin endocytic trafficking to membrane,” Weinstein Meeting, Nara, Japan, May 17, 2018. (Platform Oral Presentation)

“Cell Polarity in Cardiac Morphogenesis and Cardiac Regeneration,” Pediatric Grand Rounds, Albany Medical Center, Albany NY, Nov. 29, 2017. (Invited Speaker)

“Oriented cell division contributes to trabecular formation and trabecular specification,” The 5th Riley Heart Center Symposium on Cardiac Development. Indianapolis, IN. Nov. 19-21. (Invited Speaker)

“Numb Family Proteins regulate cardiomyocyte maturation” 2nd Meeting on Organ Regeneration with Model Organisms from Chinese Society of Cell Biology, Beijing, October 11-13, 2017 (Platform Presentation)

“Single cell lineage tracing reveals that oriented cell division contributes to trabecular morphogenesis and regional specification.” 2016 Cell Polarity Signaling (GRS), Mount Snow in West Dover, VT. June 14, 2016.

“CDC42 is required for epicardial and pro-epicardial development by regulating Fgfr1 trafficking.” Weinstein Meeting, Durham, NC. May 19, 2016. (Platform Presentation).

“Single cell lineage tracing reveals that oriented cell division contributes to trabecular morphogenesis and regional specification.” Weinstein Meeting, Durham, NC. May 19, 2016.

“Oriented Cell division Contributes to Trabecular Morphogenesis and Trabecular Differentiation.” Center of Cell Biology and Cancer Research, Albany Medical College, May 3, 2016 (Invited Talk).

“Oriented Cell division Contributes to Trabecular Morphogenesis and Trabecular Differentiation.” University of Louisville, Louisville, KY, Feb. 3, 2016. (Invited Talk)

“Decoding Congenital Heart defects at single cell resolution.” University of South Carolina, Columbia, SC. May 2015. (Invited Talk)

“Decoding Congenital Heart defects at single cell resolution.” New Jersey Medical School, Newark, NJ. May 11, 2015. (Invited talk)

“Single cell lineage tracing reveals that oriented cell division and directional migration contribute to cardiac morphogenesis.” Weinstein Meeting, Boston, MA, May 1, 2015 (Platform Presentation)

“Clonal imaging of cleared heart.” Weinstein meeting, Boston, MA. May 1, 2015 (Invited poster for Technology Fair)

“CDC42 Functions Downstream of FGF2 to Regulate Pro-Epicardial Development.” Weinstein Meeting, Boston, MA, May 2, 2015.

“Cellular dynamics of congenital heart defects via single Cell lineage tracing.” Baylor College of Medicine, Houston, TX. April 20, 2015 (Invited talk).

March 2015. Lineage tracing reveals that oriented division underlies trabecular morphogenesis and differentiation. Keystone symposia. Copper Mountain, CO.

“Decoding congenital heart defects at single cell resolution,” Fudan University, Shanghai, China. Sept. 2014 (Invited talk).

“Numb Family Proteins regulate cardiac development and cardiac function.” 2014 Cardiac Regulatory Mechanism Gordon Research Conference in New London, NH. June. 2014

“Numb family proteins are essential for cardiac morphogenesis and progenitor differentiation.” Weinstein Meeting, Tucson, AZ. May 2013 (Invited Talk)

“Cell Polarity and Cell Orientation in Cardiac Development and Disease.” Institute of Biophysics, China Academy Science, Beijing, China. June 2012 (Invited talk)

“Cell Polarity and Cell Orientation in Cardiac Development and Disease.” Nanjing University, Nanjing, China. June 2012 (Invited talk)

“Cell Polarity and Cell Orientation in Cardiac Development and Disease.” Fudan University, Shanghai, China. June 2012 (Invited talk)

“Numb family protein function in the second heart field morphogenesis and progenitor differentiation.” Weinstein Meeting, Cincinnati, OH. May 2011 (Invited talk)

“Numb Family Proteins function in cardiac development and diseases.” New York Neural Stem Cell Institute, Rensselaer, NY. March 2011 (Invited talk)

“Epicardial spindle orientation controls cell entry into the myocardium.” Maine Medical Center Research Institute, Scarborough, ME. Jan 2010. (Invited talk)