Profile: Joydip Das
Assistant Professor of Medicinal Chemistry
542 D Science and Research Building 2
Houston, TX 77204-5037
M.Sc. in Organic Chemistry, University of Burdwan, India
B.Sc. in Chemistry (with Honors), University of Calcutta, India
Postdoctoral: Medical University of South Carolina, Massachusetts Institute of Technology and Massachusetts General Hospital.
Protein Kinase C (PKC) plays a central role in signal transduction, regulating divergent cellular functions by phosphorylation of target proteins such as ion channels. Aberrations in PKC signaling have been implicated in the development of multiple human diseases including several types of cancer, metabolic and cardiovascular complications of diabetes, central nervous system dysfunctions, neuronal degeneration and cardiovascular disorders and alcoholism.
My research is directed to the following aspects of PKC:
- Alcohol Binding site in PKC epsilon- The objective of this project is to find out the alcohol binding site in PKC epsilon to understand the molecular mechanism of alcohol actions.
- Development of subtype specific PKC activators/inhibitors- Activators (diacylglycerols/phorbol esters) or the inhibitors act on more than one subtype of PKC making specific functional regulation difficult. The overall goal of this project is to develop subtype specific activators/inhibitors leading to therapeutics.
Chemical synthesis, mass spectrometry, X-ray crystallography and biochemical assays are used for this research.
- PCOL 7297-Drug design and discovery
- PHAR 5302-Medicinal Chemistry I
- PHAR 5203-Medicinal Chemistry II
- PHPS 3101-Chemical functional group analysis
- PHPS 4301-Medicinal Chemistry I
J. Das, S. Pany and A. Majhi, Chemical modification of resveratrol for improved PKC alpha activity Bioorg. Med. Chem. 19, 5321-5333 (2011)
J. Das, S. Pany, S. Panchal, A. Majhi and G.M. Rahman Binding of isoxazole and pyrazole derivatives of curcumin with the activator binding domain of novel protein kinase C. Bioorg. Med. Chem. 19, 6196-6202 (2011)
S.C. Gupta, R. Kannapan, J. Hye Kim, G. M. Rahman, S.K. Francis, R. Raveendran, M.S. Nair, J. Das and B.B. Aggarwal, Bharangin, a Diterpenoid Quinonemethide, Abolishes Constitutive and Inducible NF-kB Activation by Modifying p65 on Cysteine 38 residue and Inhibiting IkBalpha Kinase Activation, Leading to Suppression of NF-kB-Regulated Gene expression and Sensitization of Tumor Cells to Chemotherapeutic Agents., Mol. Pharmacol. 2011 (in press),
J. Das, Aliphatic diazirines as probes for proteins: recent developments, Chem. Rev. 111, 4405-17 (2011).
R. Howard, P. Slesinger, D. Davies, J. Das, R. Harris and J. Trudell, The alcohol binding site: quest for atomic level resolution, Alcohol. Clin. Ex. Res., 35, 1561-1573 (2011).
Majhi, G.M. Rahman, S. Panchal and J. Das. Binding of curcumin and its long chain derivatives to the activator binding domain of novel Protein Kinase C. Bioorg. Med. Chem. 18, 1591-1598 (2010).
J. Das, S. Pany, G. M. Rahman and S. J. Slater (2009) PKC epsilon has an alcohol binding site in its second cysteine rich regulatory domain, Biochem. J., 421, 405-413.
J. Das (2009) Photincorporation of azioctanol to the C1B domain of PKC delta is buffer dependent, J. Photochem. Photobiol B., 95, 185-8.
J. Das, X. Zhou and K. W. Miller (2006) Identification of an alcohol binding site in the first cysteine-rich domain of Protein Kinase C delta, Protein Sci. 15, 2107-2119.
J. Das, G. H. Addona, W. S. Sandberg, S. S. Husain, T. Stehle and K. W. Miller (2004) Identification of a general anesthetic binding site in the diacylglycerol–binding domain of Protein Kinase C delta, J. Biol. Chem., 279, 37964-37972.
J. Das, M. Kono, J-x. Ma, D.D. Oprian and R.K. Crouch (2004) C9 methyl group of retinal modulates the absorption and transducin activation properties in cone pigments, Biochemistry, 43, 5532-5538.
J. Ma, S. Znoiko, K.L. Othersen, J.C. Ryan, J. Das , T. Isayama, M. Kono, D.D. Oprian, D.W. Corson, M.C. Cornwall, D.A. Cameron, F.I. Harosi, C.L. Makino, R.K. Crouch (2001) A visual pigment expressed in both rod and cone photoreceptors. Neuron, 32, 451-461.
J. Ma, M. Kono, L. Xu, J. Das, J. C. Ryan, E. S. Hazard III, D. D. Oprian and R. K. Crouch (2001) Cloning, expression and spectral analysis of the Salamander UV cone visual pigment, Vis. Neuroscience, 18,393-399.
J. Das, R. K. Crouch and P. L. Chong (2000) Fluorescence properties of pyrylretinol, Photochem. Photobiol., 72, 415-422.
H. Stecher, O Prezhdo, J. Das, R. K. Crouch and K. Palczewski (1999)Isomerization of all-trans-9- and 13-desmethylretinol by retinal pigment epithelial cells, Biochemistry, 38, 13542-13550.
J. Das, R. K. Crouch, R. Govindjee and T.G. Ebrey (1999) Studies on the Pyryl retinal analogues of bacteriorhodopsin, Photochem. Photobiol., 70, 949-956.
S. Panchal, A. Majhi, V. Tam and J. Das, Synthesis of Poly-Phenolic Derivatives and Evaluation for Anti-Bacterial and Anti-Fungal Activity, University of Houston Research Day Poster session, 2009.
J. Das and G. M. Rahman, Development of alcohol antagonists based on Protein Kinase C epsilon, Alcohol. Clin. Exp. Res., 33(6), 2009, suppl. Issue, p17A
A. Majhi, S. Panchal, G.M. Rahman and J. Das., Interaction protein Kinase C and Curcumin and its derivatives, Thirty-Sixth Annual MALTO, Memphis, May 17-19, 2009
A. Guillory, S. Pany and J. Das, Alcohol binding site(s) in the C1 domain of MUNC 13.1, Thirty-Sixth Annual MALTO, Memphis, May 17-19, 2009
G.M. Rahman and J. Das., Virtual molecular docking study of DAG and phorbol ester-like compounds to explore the differential binding affinity of C1A and C1B subdomains of Novel PKCs, Thirty-Sixth Annual MALTO, Memphis, May 17-19, 2009
A. Guillory, S. Pany and J. Das, Alcohol binding site(s) in the C1 domain of PKC epsilon and MUNC 13.1, Behavior, Biology, Chemistry: Translational Research in Addiction, San Antonio, TX March 21-22, 2009 (Travel Award to A. Guillory).
J. Das, Role of histidine and tyrosine residues in the alcohol binding in Protein Kinase C Epsilon, Alcohol. Clin. Exp. Res., 32(6), 2008, suppl. Issue, p161A.
J. Das, S. Shunmugasundararaj, T. Stehle, and K. W. Miller. Crystal structure of an alcohol complexed with the cysteine-rich domain of Protein Kinase C delta. Alcohol. Clin. Exp. Res., 2007, 31(6), suppl. Issue, p202A
J. Das. Investigation on the alcohol binding site(s) in the cysteine-rich domain of Protein Kinase C epsilon. Alcohol. Clin. Exp. Res., 2007, 31(6), suppl. Issue, p195A.
J. Das and K. W. Miller. In the C1 domain of Protein Kinase C delta both C1A and C1B contain an alcohol binding domain. Biophys. J. 2006; 90(1):40a.
J. Das and K. W. Miller. Identification of an alcohol binding site in the first cysteine rich domain of protein kinase C delta by photolabeling and mass spectrometry. Biophys. J. 2005; 88(1):45a
Invited Lectures and Seminars
Invited Talk at the University of Wisconsin, Eau Claire, Oct. 7, 2011, titled “Alcohol Binding Sites in Brain Proteins.
Jadavpur University, Calcutta, India, January, 2008.
The University of Texas at El Paso, Texas, November, 2007
Indiana Centers for Applied Protein Sciences, Indianapolis, Indiana, October, 2005
Curagen Corporation, Branford, Connecticut, March, 2004
- American Chemical Society
- Protein Society
- Research Society on Alcoholism
- Biophysical Society
- American Society for Photobiolog