Profile; Romi Ghose

Romi Ghose
Romi Ghose, Ph.D.
Assistant Professor of Pharmaceutics
Department of Pharmacological and Pharmaceutical Sciences
Texas Medical Center
1441 Moursund Street, Rm 517
Houston, TX 77030
Phone: 832-842-8343

Fax: 832-842-8305
rghose@uh.edu

Education

BSc. (Chemistry), University of Calcutta, India
MSc. (Biochemistry), University of Calcutta, India
Ph.D (Biochemistry), University of Notre Dame, Notre Dame, IN
Post-doctoral, Baylor College of Medicine, Houston, TX

Research Interests

Regulation of drug metabolism and toxicity

Drug-metabolizing enzymes regulate metabolism of drugs and toxins, and are critical to the survival of mammalian species. Alterations of these enzymes can lead to impaired metabolism of drugs, leading to adverse drug reactions which can have deleterious consequences in terms of human health. Our long-term goal is to increase the efficacy and safety of drugs by characterization of these enzymes at a molecular level.

Our current research focuses on investigating the role of inflammation, infectious diseases and obesity on drug-metabolizing enzymes and pharmacokinetics and hepatotoxicity of candidate drugs. Biochemical assays, molecular biology techniques and transgenic animal models are utilized in our studies. The knowledge gained from these studies will have significant impact on developing novel strategies to maximize therapeutic efficacy of drugs and reduce incidences of drug-induced liver disease.

Courses

  • PCEU 6342: Advanced Physical Pharmacy I
  • PCOL 7350: Cellular Pharmacology
  • PHAR 4331: Pharmaceutics II

Research Grants

Completed
  • K01 DK076057-02K01. National Institute of Diabetes and Digestive and Kidney Diseases. "Hepatic drug metabolism in inflammation." 2006-2010.
  • Small Grants Program. University of Houston. "Regulation of hepatic drug metabolism in obesity." 2008-2010.  Romi Ghose, PI
  • Pilot/Feasibility Award in GI Research, Texas Gulf Coast Digestive Disease Center.  “Endobiotic/Xenobiotic metabolism in inflammation.” 2005-2006. Romi Ghose, PI.

Selected Publications

Gandhi A, Guo T, Shah P, Moorthy B, Ghose R. Chlorpromazine-induced hepatotoxicity during inflammation is mediated by TIRAP-dependent signaling pathway in mice. Toxicol Appl Pharmacol. 2013 Feb 1;266(3):430-8. doi: 10.1016/j.taap.2012.11.030.

Fahy BN, Guo T, Ghose R. Impact of hepatic malignancy on CYP3A4 gene expression. J Surg Res. 2012 Dec;178(2):768-72. doi: 10.1016/j.jss.2012.06.008.

Gandhi A, Moorthy B, Ghose R. Drug disposition in pathophysiological conditions. Curr Drug Metab. 2012 Nov;13(9):1327-44. Review.

Gandhi AS, Guo T, Shah P, Moorthy B, Chow DS, Hu M, Ghose R. CYP3A-dependent drug metabolism is reduced in bacterial inflammation in mice. Br J Pharmacol. 2012 Aug;166(7):2176-87. doi: 10.1111/j.1476-5381.2012.01933.x.

Ghose R, Guo T, Vallejo JG, Gandhi A. Differential role of toll-interleukin 1 receptor domain-containing adaptor protein in toll-like receptor 2-mediated regulation of gene expression of hepatic cytokines and drug-metabolizing enzymes. Drug Metab Dispos. 2011 May;39(5):874-81.

Ghose R, Omoluabi O, Gandhi A, Shah P, Strohacker K, Carpenter KC, McFarlin B, Guo T. Role of high-fat diet in regulation of gene expression of drug metabolizing enzymes and transporters. Life Sci. 2011 Jul 4;89(1-2):57-64.

Adarsh Gandhi, Tao Guo and Romi Ghose. Role of c-jun N-terminal kinase in regulating tumor necrosis factor-alpha mediated increase of acetaminophen and chlorpromazine toxicity in murine hepatocytes. J. Toxicol. Sci. 2010;35(2):163-73.

Tam VH, Ledesma KR, Schilling AN, Lim TP, Yuan Z, Ghose R and Lewis RE. In vivo dynamics of carbapenem-resistant Pseudomonas aeruginose selection following suboptimal dosing Diagnostic Microbiology and Infectious Disease. Diagn Microbiol Infect Dis 2009, 64; 427-433.

Ghose R, Guo T, Haque N. Regulation of gene expression of hepatic drug metabolizing enzymes and transporters by the Toll-like receptor 2 ligand, lipoteichoic acid. Arch. Biochem. Biophys. 2009, 481 (1): 123-30.

Ghose R, White D, Guo T, Vallejo S, Karpen SJ. Regulation of hepatic drug metabolizing enzyme genes by Toll-like receptor 4 signaling is independent of TIR-domain containing adaptor protein (TIRAP). Drug Met. Disp. 2008; 36 (1): 95-101.

Ghose, R., J. Mulder and S. J. Karpen.  Lipoplolysaccharide-mediated down-regulation of hepatic genes is ameliorated by the PPARg agonist, Rosiglitazone. J Hepatol. 2007 46 (1): 115-123.

Zimmerman T.L, Thevananther S, Ghose R, Burns A and S. J. Karpen.  Nuclear export of RXR in response to IL-1b-mediated cell signaling: roles for JNK and Ser260. J. Biol. Chem. June 2, 281 (22): 15434-40, 2006.

Abstracts

Pranav Shah, Ozozoma Omoluabi and Romi Ghose. Toll-Like Receptor 3-Mediated Regulation of Drug Metabolizing Enzymes and Transporters: Role of TRIF-Dependent Signaling. American Association of Pharmaceutical Scientists (AAPS); Chicago, October 2012. 

Adarsh Gandhi, Pranav Shah and Romi Ghose.  Altered metabolism of irinotecan (CPT-11) in diet-induced obese mice. Annual Conference of Texas Screening Alliance for Cancer Therapeutics (TxSACT 2012); Houston, TX.

Mary Shenouda, Pranav Shah and Romi Ghose.  Role of CAR in regulation of phase II drug metabolizing enzymes.  University of Houston Undergraduate Research Day, 2012.

Pranav Shah, Tao Guo and Romi Ghose. Regulation Of Drug Metabolizing Enzymes & Transporters by Toll-Like Receptor-2 Is Mediated By CAR. 25th Annual meeting of the American Association of Pharmaceutical Scientists. Washington D.C., 2011.

Adarsh Gandhi, Tao Guo, Pranav Shah and Romi Ghose. Toll-like receptor signaling adaptor protein, TIRAP, regulates the hepatotoxic responses of chlorpromazine in inflammation. The 62nd Annual meeting of the American Association for the Study of Liver Diseases (AASLD 2011); San Francisco, CA. 

Adarsh Gandhi, Tao Guo, Ming Hu, Diana Chow and Romi Ghose. Midazolam metabolism is compromised in Inflammation. Experimental Biology, Washington DC, April 2011.

Adarsh Gandhi, Tao Guo and Romi Ghose. Role of Toll-interleukin 1 receptor domain containing adaptor protein (TIRAP) and c-Jun-N-terminal kinase (JNK) in hepatotoxicity of chlorpromazine. 24th Annual meeting of the American Association of Pharmaceutical Scientists. New Orleans, LA. 2010.

Adarsh Gandhi, Tao Guo and Romi Ghose. Midazolam metabolism is compromised in Inflammation. 16th International Conference. Inflammation Research Association. Chantily, VA, 2010.

Omoluabi, O., Guo, T and Ghose, R. Toll-like receptor 3: Novel regulators of drug metabolism.  Gordon research Conference on Drug Metabolism, Holderness School, New Hampshire, July 2010.

Ghose, R. and Tao Guo. Role of Toll-like receptor signaling in hepatocytes in regulating the gene expression of drug metabolizing enzymes. Experimental Biology, ASPET, Anaheim, CA, 2010.

Ghose, R., Tao Guo and Adarsh Gandhi. Toll-like receptors: novel regulators of hepatic drug metabolism. AASLD, Boston, MA, November 2009.

Ghose, R., Tao Guo and Adarsh Gandhi. Role of Toll-like receptors and the adaptor protein, TIRAP in regulation of gene expression of hepatic drug metabolizing enzymes. AASLD Single Topic Conference, Atlanta, Georgia, September 2009.

Romi Ghose, Tao Guo and Adarsh Gandhi. Role of Toll-like receptor adaptor protein, TIRAP in regulation of gene expression of drug metabolizing enzymes. Division of Drug Metabolism Platform Session. Experimental Biology, ASPET, New Orleans, LA, April 2009.

Adarsh Gandhi, Tao Guo, Gaurav Dhar and Romi Ghose. Role of cytokines in regulating drug-induced hepatotoxicity. Frontiers in Digestive Diseases Symposium, Houston, TX, February 2009.

Adarsh Gandhi, Tao Guo and Romi Ghose. Lipopolysaccharide and cytokine-induced drug hepatotoxicity. AAPS, Atlanta, GA, November 2008.

Romi Ghose, Tao Guo, Nadia Haque and Adarsh Gandhi. Role of Toll-like receptor 2 signaling pathway in regulating the gene expression of hepatic drug metabolizing enzymes. Gordon Research Conference on Drug Metabolism, Holderness School, NH, July 2008.

Romi Ghose and Tao Guo. Lipoteichoic acid modulates hepatic drug metabolizing enzyme gene expression by activating the Toll-like receptor 2 signaling pathway. Experimental Biology, ASPET, San Diego, CA, April 2008.

V.H. Tam, K.R. Ledesma, A.N. Schilling, K.T. Chang, T.P. Lim, R. Ghose, R.E. Lewis. In-vivo selection of carbapenem-resistant Pseudomonas aeruginosa following sub-optimal dosing. European Society of Clinical Microbiology and Infectious Diseases. Barcelona, Spain, April 2008.

Ghose, R., White, D. A., Guo, T., Vallejo, J., and S. J. Karpen. Role of the Toll-like receptor signal transduction pathway in regulation of drug metabolizing enzymes in inflammation. GTCbio Annual Conference on Protein Kinases in Drug Discovery, Boston, MA, 2007.

Ghose, R., White, D. A., Guo, T., Vallejo, J., and S. J. Karpen. Regulation of drug metabolizing enzymes in inflammation: Role of the Toll-like receptor 4 signaling pathway. Experimental Biology, ASPET, Washington, D.C., 2007.

Sun H, Ghose R, Karpen S.J. and Thevananther, S. Hepatocyte proliferative response to partial hepatectomy is markedly impaired in TIRAP Knockout mice. Hepatology, Vol 46, No. 4, Suppl. 1, 531A, 2007.

Ghose, R., Doerre, D. A., Vallejo, J., and S. J. Karpen. TLR4, but not the TLR adaptor protein, TIRAP, is required for LPS-mediated suppression of drug metabolizing enzyme (DME) gene expression. Hepatology, (Suppl.), 44, 2006.

Kosters, A., Gulati, A., Thevananther, S., Mulder, J., Von Furstenberg, R., Zimmerman, T. L., Ghose, R., and S. J. Karpen. Dietary curcumin prevents downregulation of hepatic transporter gene expression by LPS. Hepatology, (Suppl.), 42, 570A, 2005.

Ghose, R., J. Mulder, Von Furstenberg, R., and S. J. Karpen. Rosiglitazone attenuates LPSmediated suppression of RXR-alpha-regulated hepatic genes. Hepatology, (Suppl.), 42, 568A, 2005.

Zimmerman T. L., Ghose, R., Thevananther, S., Kosters, A., Von Furstenberg, R., and S. J. Karpen. IL-1 beta induced ubiquitination and proteasomal degradation of Retinoid X Receptor alpha in HepG2 cells. Hepatology, (Suppl.), 42, 567A, 2005.

Oral Presentations

Ghose, R.  Toll-like receptors: Novel regulators of hepatic drug metabolizing enzymes. Drug Metabolism Platform Session: Experimental Biology, Washington DC, April, 2011.

Adarsh Gandhi, Tao Guo, Ming Hu and Romi Ghose. Inflammation-induced hepatotoxicity of chlorpromazine is mediated by the Toll-interleukin 1 receptor adaptor protein (TIRAP). Annual conference of Gulf Coast Chapter Society of Toxicology, Houston, TX, 2010.

Ghose, R., and Tao Guo. Role of Toll-like receptor adaptor protein, TIRAP in regulation of gene expression of drug metabolizing enzymes. Division of Drug Metabolism Platform Session. Experimental Biology, ASPET, New Orleans, LA, April 2009.

Ghose, R., T. Guo, A. Gandhi, S. J. Karpen and D. White. Role of Toll-like receptors on hepatocytes in regulating the gene expression of drug metabolizing enzymes. Parallel Session on Drug and Alcohol Toxicity: The 59th Annual meeting of the American Association of Liver Diseases, San Francisco, CA, 2008.

Ghose, R. Liver toxicity consideration in drug discovery and development. ACS Southwest Regional Meeting Symposium, 2006.

Invited Lectures

Drug Efficacy and Safety in Obesity. Texas Obesity Research Center Conference, Houston, Texas, April, 2011.

Biopharmaceutical Considerations in Drug Development. Texas Obesity Research Center Conference, Houston, Texas, November, 2010.

Regulation of Hepatic Drug Metabolizing Enzymes in Inflammation. Texas Medical Center Digestive Diseases Center, Frontiers in Digestive diseases Symposium, Houston, TX, April, 2010. 

Toll-like receptor mediated regulation of hepatic drug metabolism. Institute of Biosciences and Technology, Texas A&M University, Houston, TX, December 2009.

Hepatic Drug metabolism in Inflammation. College of Pharmacy, University of Texas at Austin, Austin, TX, September 2009.

Inflammation and Hepatic Drug Metabolism. Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, Kolkata, India, January 2008.

Inflammation and Hepatic Drug Metabolism. Genitourinary Oncology Program Seminar Series. The Methodist Hospital. August 2007.

Molecular mechanism of regulation of drug metabolism and toxicity. New Investigator Symposium, Houston, TX, May 2007.

Mechanisms underlying altered endobiotic/xenobiotic metabolism in inflamed liver. Feigin Center Symposium, Baylor College of Medicine, Houston, TX, January 2005.

Memberships

  • American Association for the Study of Liver Diseases
  • American Society for Pharmacology and Experimental Therapeutics
  • International Society for the Study of Xenobiotics
  • Society of Toxicology

Editorial Appointments

  • Manuscript Reviewer (ad hoc): Journal of Hepatology, Hepatology, European Journal of Pharmacology, British Journal of Pharmacology, Life Sciences