BCHS 3304 Fall 2001
Homework #4

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Basic Calculations, Protein Structure and Purification

Note:
This homework will not be collected. However, quizzes and exams will assume that you have completed and understand the homework assignment and could answer related questions.
Reading Assignment: Chapter 6 of Biochemistry
Chapter 5, Sect. 2. pages 96-107 of Biochemistry
Study Exercises: Chapter 6, p. 159, #1, 3, 7, 9, 11, 12
Chapter 5, p. 122, #2-4
Show all work and remember to incorporate your units in your calculations.
  1. Your experiment requires 100 ml 20 mM Tris, pH 8.0 which also contains 0.5 mM EDTA. Calculate and describe how you would make this solution using a stock of 1 M Tris, pH 8.0 and 0.5 M EDTA, pH 8.0.

  2. Valine and isoleucine are isosteric indicating that they have the same shape. Draw each of these amino acids. What are their three-letter codes? What are their one-letter codes? Draw a circle around the methyl group which distinguishes isoleucine and valine.

  3. Identify and draw another pair of isosteric amino acids in addition to the valine and isoleucine pair.

  4. Complete Problem 1 of Chapter 6 (p. 75) in the Student Companion to Biochemistry.

  5. Complete Problem 2 of Chapter 6 (p. 75) in the Student Companion to Biochemistry.

  6. Complete Problem 4 of Chapter 6 (p. 76) in the Student Companion to Biochemistry.

  7. Complete Problem 13 of Chapter 6 (p. 77) in the Student Companion to Biochemistry.

  8. Complete Problem 14 of Chapter 6 (p. 77) in the Student Companion to Biochemistry.

  9. Complete Problem 18 of Chapter 6 (p. 77) in the Student Companion to Biochemistry.

  10. Complete Problem 20 of Chapter 6 (p. 77) in the Student Companion to Biochemistry.

  11. Complete Problem 24 of Chapter 6 (p. 78) in the Student Companion to Biochemistry.

  12. Complete Problem 26 of Chapter 6 (p. 79) in the Student Companion to Biochemistry.

  13. Complete Problem 1 of Chapter 6 (p. 159) in your Biochemistry textbook.

  14. Complete Problem 2 of Chapter 6 (p. 159) in your Biochemistry textbook.
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