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Review of Basic Calculations, Life, Thermodynamics, and
Water:
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Note:
This homework will not be collected. However, quizzes and exams
will assume that you have completed and understand the homework
assignment and can answer related questions.
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Reading Assignment: |
Chapter 1 of Biochemistry
Chapter 2 of Biochemistry. |
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Study Exercises: |
Chapter 1, p. 21, #4-6
Chapter 2, p. 38, #1-4 |
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Show all work and
remember to incorporate your units throughout your calculations. |
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- You have just begun your Senior Honor's Thesis. Your advisor
asks you to make a series of stock solutions. She also explains
that you may use a pH meter to adjust the pH. Calculate how
much of the solid reagent you would add to make the following:
A. 500 ml 1 M Tris, pH 8.0 (MW=121.4 g/mole)
B. 1.0 L 5 M NaCl (MW= 58.44 g/mole)
C. 10 ml 100 mg/ml ampicillin (MW=371.4 g/mole)
D. 500 ml 0.5 M EDTA (ethylene diamine tetra-acetic acid;
MW=292.2 g/mol)
E. 200 ml 1 M MgCl2 (Note: MgCl2 is sold by the chemical company
as co-crystallized with H20. Thus, the MW of MgCl2 6H20 is
203.30 g/mole)
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For your first experiment, you need to make a solution that
is commonly called TE and stands for Tris-EDTA. It is comprised
of 10 mM Tris, 1 mM EDTA, pH 8.0. Calculate and describe how
you would make 100 ml of TE using the stock solutions that
you have already made above in #1.
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Complete Problem 1 of Chapter 1 (p. 5) in the Student Companion
to Biochemistry.
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Complete Problem 7 of Chapter 1 (p. 6) in the Student Companion
to Biochemistry.
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Complete Problem 8 of Chapter 1 (p. 6) in the Student Companion
to Biochemistry.
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Complete Problem 9 of Chapter 1 (p. 6) in the Student Companion
to Biochemistry.
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Complete Problem 12 of Chapter 1 (p. 7) in the Student Companion
to Biochemistry.
- Complete Problem 13 of Chapter 1 (p. 7) in the Student Companion
to Biochemistry.
- Complete Problem 15 of Chapter 1 (p. 7) in the Student Companion
to Biochemistry.
- Complete Problem 18 of Chapter 1 (p. 7) in the Student Companion
to Biochemistry.
- Complete Problem 1 of Chapter 1 (p. 21) in your Biochemistry
textbook.
- Complete Problem 2 of Chapter 1 (p. 21) in your Biochemistry
textbook.
- Complete Problem 5 of Chapter 1 (p. 21) in your Biochemistry
textbook.
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