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Basic Calculations, Amino Acids
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Note:
This homework will not be collected. However, quizzes and exams
will assume that you have completed and understand the homework
assignment and could answer related questions.
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Reading Assignment: |
Chapter 5 of Biochemistry (except Sec.
2 pages 96-107) |
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Study Exercises: |
Chapter 5, p. 122, #6-7 |
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Show all work and remember to incorporate
your units in your calculations.
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- You are purifying the protein called myoglobin which has
been expressed recombinantly in the bacteria E. coli. In order
to release the protein, you need to add the enzyme lysozyme
to break down the bacterial cell wall. Your experiment calls
for 10 mg of lysozyme and recommends that you make 10 ml of
a 50 mg/ml stock solution of lysozyme in TE buffer. The molecular
weight of lysozyme is 14,300 g/mole. Calculate and describe
how you would make your stock solution. Calculate what volume
of your stock solution you would add to your protein purification
experiment
- Your thesis advisor suggests that you make stock solutions
for your protein gel electrophoresis experiment to look at your
purified myoglobin. She tells you the components for 1x Tris-Glycine
electrophoresis buffer, but suggests you actually make a 1.0
L 5x stock so that you simply can dilute the concentrate as
you need it. Calculate and describe how you would make a 5x
stock of Tris-Glycine electrophoresis buffer. (Hint: 1% = 1g/100
ml for an aqueous solution.)
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1x Tris-Glycine electrophoresis buffer
(25 mM Tris, 250 mM Glycine, 0.1% SDS)
Tris MW=121.4 g/mole
Glycine MW= 75.07 g/mole
SDS (sodium dodecyl sulfate) MW=288.38 g/mole |
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- Your SDS-PAGE gel electrophoresis experiment
requires 1.0 L of 1x Tris-Glycine buffer. Calculate and describe
how you would make this solution using your 5x stock solution.
- Name two amino acids that would be found in the core of a
protein. What are their three-letter codes? What are their one-letter
codes?
- Name one hydrophobic amino acid. What is its three-letter
code? What is its one-letter code?
- Name two basic amino acids. What are their three-letter codes?
What are their one-letter codes?
- Name two amino acids that might be found on the surface of
a protein. What are their three-letter codes? What are their
one-letter codes?
- Name two acidic amino acids. What are their three-letter codes?
What are their one-letter codes?
- Complete Problem 15 of Chapter 5 (p. 61) in the Student
Companion to Biochemistry.
- Complete Problem 16 of Chapter 5 (p. 61) in the Student
Companion to Biochemistry.
- Complete Problem 17 of Chapter 5 (p. 61) in the Student
Companion to Biochemistry.
- Complete Problem 20 of Chapter 5 (p. 61) in the Student
Companion to Biochemistry.
- Complete Problem 21 of Chapter 5 (p. 62) in the Student
Companion to Biochemistry.
- Complete Problem 7 of Chapter 5 (p. 122) in your Biochemistry
textbook.
- Complete Problem 8 of Chapter 5 (p. 123) in your Biochemistry
textbook
- Complete Problem 9 of Chapter 5 (p. 123) in your Biochemistry
textbook.
- Complete Problem 12 of Chapter 5 (p. 123) in your Biochemistry
textbook.
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