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Basic Calculations, Amino Acids
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Note:
This homework will not be collected. However, quizzes and
exams will assume that you have completed and understand the homework
assignment and could answer related questions.
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Reading Assignment: |
Reading Assignment: Chapter 4 of Biochemistry. |
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Study Exercises: |
Chapter 4, p. 92, #1-3 |
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Memorization Assignment: |
You should be able to draw the structures of each
of the twenty amino acids.
You should know the 3 letter and 1 letter code for each amino
acid.
You do NOT have to memorize structures for non-standard amino
acids.
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Show all work and remember to incorporate
your units in your calculations.
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- Your advisor asks you to make two concentrated stock solutions
for the whole laboratory to use. She explains that it is common
to make a concentrated solution to dilute for use. Thus, a 10x
solution refers to one that is 10-fold concentrated. Calculate
how you would make 1.0 L of each of the following:
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A. 10x TBE ( 0.9 M Tris, 0.9 M Boric
Acid, 20 mM EDTA |
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Tris MW=121.4 g/mole
Boric Acid MW=61.84 g/mole
EDTA MW=292.2 g/mole |
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B. 50x TAE (2 M Tris, 2 M Acetic acid,
0.5 M EDTA) |
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Tris MW=121.4 g/mole
Concentrated Acetic acid (glacial) comes as a solution and is 17.4
M
EDTA MW=292.2 g/mole |
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- Your polyacrylamide gel electrophoresis experiment
requires 1.0 L of 0.5x TBE. Calculate and describe how you would
make this solution using your stock solutions.
- Your agarose gel electrophoresis experiment requires 500 ml
of 1x TAE. Calculate and describe how you would make this solution
using your stock solutions.
- Draw the structure for Methionine. What is its three-letter
code? What is its one-letter code?
- Draw the structure for Cysteine. What is its three-letter
code? What is its one-letter code?
- Complete Problem 2 of Chapter 4 (p. 40) in the Student Companion
to Biochemistry.
- Complete Problem 5 of Chapter 4 (p. 41) in the Student Companion
to Biochemistry.
- Complete Problem 6 of Chapter 4 (p. 41) in the Student Companion
to Biochemistry.
- Complete Problem 7 of Chapter 4 (p. 41) in the Student Companion
to Biochemistry.
- Complete Problem 8 of Chapter 4 (p. 41) in the Student Companion
to Biochemistry.
- Complete Problem 13 of Chapter 4 (p. 43) in the Student Companion
to Biochemistry.
- Complete Problem 14 of Chapter 4 (p. 43) in the Student Companion
to Biochemistry.
- Complete Problem 17 of Chapter 4 (p. 43) in the Student Companion
to Biochemistry.
- Complete Problem 18 of Chapter 4 (p. 44) in the Student Companion
to Biochemistry.
- Complete Problem 1 of Chapter 4 (p. 92) in your Biochemistry
textbook.
- Complete Problem 2 of Chapter 4 (p. 92) in your Biochemistry
textbook.
- Complete Problem 5 of Chapter 4 (p. 92) in your Biochemistry
textbook.
- Complete Problem 6 of Chapter 4 (p. 92) in your Biochemistry
textbook.
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