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Highly Specific Assays

Detecting  nucleic  acids  and  proteins  have  been  is  made  possible  by  the  historic  and  revolutionary  technologies  like  Polymerase  Chain  Reaction  (PCR)  and  ELISA.  PCR currently cannot be applied to non-nucleic biomolecules and ELISA has limited sensitivity. Immuno-PCR is another powerful technology that combines PCR and ELISA.  This technology   is   an   advancement   over   the   immuno-PCR   detection   technique   using   DNA- Avidin in reporter nanoparticles.  The multi template DNA-Avidin nanoparticles created have specific sensitivity to multiple biotin binding sites. The assay results in 100X more sensitivity compared to currently used immuno-assays.

Applications

  • Detection of specific non-nucleic biomolecules including hormones, biomarkers, toxins etc.

Problem Addressed

  • PCR is a highly sensitive assay, however it only works in the presence of nucleic acids
  • ELISA is applicable for hormone, antibodies, proteins toxins, however has lower sensitivity than PCR
  • Immuno-PCR combines the specificity of an ELISA with the signal amplification of PCR. However, its sensitivity is not adequate for the detection of analytes of low abundance
  • Immuno-PCR is a complex and expensive assay

Competitive Advantages

  • Highly sensitive and applicable to multiple biomolecules
  • Avoids antibody-DNA conjugation
  • Assay does not require fresh tissue biopsy

Patents

  • Provisional patent filed