Dr. Riad Efendiev

College of Pharmacy, University of Houston,
4800 Calhoun, Houston, TX 77204
tel: (713) 743 1228,
fax: (713) 743 1229,
e-mail: efendiev@jetson.uh.edu

Area of Expertise:

Experimental Biochemistry, Cell Biology, Molecular Cloning.

Education:

December 1996, PhD in Biochemistry.

Moscow University of Physics & Technology,
Dept. of Physical & Chemical Biology, Moscow, Russia.
Thesis title: "The Na,K-ATPase topography study by surface-specific labeling".

June 1992, MS in Molecular Biology.

Moscow University of Physics & Technology,
Dept. of Physical & Chemical Biology, Moscow, Russia.

Employment:

Since March 1999
Research Associate 2,
Dept. of Pharmacological & Pharmaceutical Sciences,
College of Pharmacy, University of Houston, Houston, Texas

Study in progress.

September 1997 - February 1999
Postdoctoral Fellow 2,
Dept. of Pharmacological & Pharmaceutical Sciences,
College of Pharmacy, University of Houston, Houston, Texas

  • Transfection and purification of Na,K-ATPase from transfected cells.
  • Study of functional changes in mutant enzyme with the response to phorbol esters, dopamine and epinephrine.
  • Study of Na-pump stimulation inhibition dependency on PKC isozymes involved.

Results:

  • a model of ATPase activity and ion transport dependence on phosphorylation was proposed;
  • PKC-beta and PKC-zeta were found to mediate respectively, phorbol ester stimulation and dopamine inhibition of the Na-pump.

February - August 1997
Postdoctoral Fellow 1,
Dept. of Pharmacological & Pharmaceutical Sciences,
College of Pharmacy, University of Houston, Houston, Texas

  • Purification of Na,K-ATPase from transfected cells.
  • Study of functional changes in mutant enzyme, treated with various hormones and drugs.
  • Cross-linking application for topological model.

Result:

  • Ser-11 and Ser-18 residues were found to be phosphorylation sites of Na,K-ATPase.

1996-1997
Junior Research Scientist,
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia

  • Study of Na,K-ATPase stucture-function relationships.

Result:

  • Tyrosine amino acid residue was found to be a component of Na,K-ATPase active center.

1990-1995
Research Assistant,
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia

  • Purification and characterization of Na,K-ATPase isoforms from various tissues.
  • Covalent modification of amino acid residues.
  • Transmembrane topological model development.

Result:

  • Na,K-ATPase topological model with even number of transmembrane segments was developed.

Honors:

1996Travel grant to the 10th Annual Simposium of the Protein Society, San Jose, CA.
1995 & 1996International Soros Scientific Educational Program grant for graduate students.

Languages:

English and Russian fluent, Azeri and French basic.

References:

  1. Dr. Carlos H. Pedemonte, Associate Professor, College of Pharmacy, University of Houston, 4800 Calhoun, Houston, TX 77204, tel: (713) 743 1211, fax: (713) 743 1229, e-mail: pedemonte@jetson.uh.edu.
  2. Dr. Alejandro M. Bertorello, Associate Professor, Dept. of Molecular Medicine, Karolinska Hospital, S-171 76 Stackholm, Sweden, tel: (46 8) 517 75727, fax: (46 8) 517 73658, email: alejan@enk.ks.se.
  3. Dr. Nikolai N. Modyanov, Professor, Dept. of Pharmacology, Medical College of Ohio, 3000 Arlington Ave, Toledo, OH 43614, tel: (419) 383 4182, fax: (419) 383 2871, e-mail: nmodyanov@cutter.mco.edu.
  4. Dr. Natalia M. Vladimirova, Senior Researcher, Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, 16/10 Miklukho-Maklaya, 117871 Moscow, Russia, tel: (7 095) 330 6647, fax: (7 095) 330 6456, e-mail: vla@ibch.siobc.ras.ru.

Articles:

  1. Vladimirova, N.M., Efendiyev, R.E., Potapenko, N.A., Modyanov, N.N. Determination of sidedness of COOH-terminus of Na/K-ATPase alpha subunit. (1994) in The Sodium Pump. Structure, Mechanism, Hormonal Control and its Role in Decease (Eds. E. Bamberg, W. Schoner), Darmstadt, 370-373.
  2. Vladimirova, N., Efendiev, R., Platoshkina, E., Potapenko, N., Modyanov, N. Lactoperoxidase iodination of pig kidney Na,K-ATPase. (1995) Membrane & Cell Biology (Moscow), 8, 615-624.
  3. Vladimirova, N.M., Efendiyev, R.E., Lakhtina, O.E., Potapenko, N.A., Modyanov, N.N. Application of lactoperoxidase iodination method for topographical study of the Na,K-ATPase catalytic subunit. (1996) Membrane & Cell Biology (Moscow), 9, 27-34.
  4. Vladimirova, N.M., Platoshkina, E.A., Efendiyev, R.E., Potapenko, N.A. Isolation, structural and functional analysis of new types of Na,K-ATP-ase isozymes. (1997) Annals of the New York Academy of Sciences, 834, 153-155.
  5. Vladimirova, N., Platoshkina, E., Efendiyev, R., Potapenko, N. The study of 3D surface of the sodium pump and its conformational changes. (1998) Annals of the New York Academy of Sciences, in press.
  6. Efendiev, R., Bertorello, A., Pedemonte, C. PKC-beta and PKC-zeta mediate opposing effect on proximal tubule Na,K-ATPase activity. (1999) FEBS Letters, 456, 45-48.
  7. Efendiev, R., Bertorello, A., Pressley, T., Rousselot, M., Beguin, P., Feraille, E., Pedemonte, C. Stimulation of Na,K-ATPase activity by phorbol esters is mediated by phosphorylation of alpha-subunit Ser-11 and Ser-18, and recruitment of transport molecules to the plasma membrane. J. Biol. Chem. in preparation.
  8. Yudowski, G.A., Efendiev, R., Pedemonte, C., Katz, A., Berggren, P.-O., Bertorello, A. Phosphatidylinositol-3'kinase p85a subunit binds to a proline-rich motif in the Na,K-ATPase a-subunit and regulates its trafficking. Science in preparation.

Abstracts:

  1. Vladimirova, N., Efendiyev, R., Potapenko, N., Modyanov, N. Determination of sidedness of COOH-terminus of Na,K-ATPase alpha subunit by vectorial labeling. (1993) Biol. Chem. Hoppe-Seyler, 374, p. 581.
  2. Vladimirova, N.M., Efendiyev, R.E., Platoshkina, E.A., Potapenko, N.A. Isolation, structural and functional analysis of new types of Na,K-ATP-ase isozymes. International Workshop 'Membrane Bioenergetics', Moscow, Russia, 15-20 December 1995.
  3. Vladimirova, N.M., Efendiyev, R.E. Iodination of Na,K-ATP-ase tyrosine residue(s) induces inhibition of enzyme activity. (1996) FASEB Journal, 10, A651.
  4. Vladimirova, N.M., Efendiyev, R.E. Lactoperoxidase-mediated radioiodination as an approach for the detection of tyrosine residues exposition on the protein globule surface. 'Protein Folding and Design' Conference, Bethesda, MD, 23-26 April 1996.
  5. Efendiyev, R.E., Platoshkina, E.A., Vladimirova, N.M. Surface-specific modification of Na,K-ATP-ase tyrosine residues. 10th Annual Simposium of the Protein Society, San Jose, CA, 3-7 August 1996.
  6. Vladimirova, N.M., Platoshkina, E.A., Efendiyev, R.E., Potapenko, N.A. Isolation, structural and functional analysis of new types of Na,K-ATP-ase isozymes. 8th International Conference on Na/K-ATPase, Mar del Plata, Argentina, 26-30 August 1996.
  7. Efendiev, R., Pressley, T., Bertorello, A., Pedemonte, C. Amino acids involved in PKC mediated stimulation of Na-pump activity in renal cells. Mol Biol. Cell, 1997, 8, 84a.
  8. Efendiev, R., Pressley, T., Bertorello, A., Pedemonte, C. Relevance of the a-subunit Ser-11 and Ser-18 to phorbol ester regulation of kidney epithelial Na-pump. (1998) Biophys. J., 74, A196.
  9. Efendiev, R., Pressley, T., Bertorello, A., Pedemonte, C. Stimulation of PKC produces both activation and inhibition of Na-pump activity in renal epithelial cells. (1998) FASEB J., in press.
  10. Efendiev, R., Bertorello, A., Pressley, T., Pedemonte, C. Molecular mechanism responsible for hormonal regulation of renal Na-pump. (1999) Biophys. J. in press.
  11. Yudowski, G.A., Pedemonte, C., Efendiev, R., Katz, A., Bertorello, A. Phosphatidylinositol-3'kinase p85a subunit binds to a proline-rich motif in the Na,K-ATPase a-subunit and regulates its endocytosis. 32nd Annual meeting of ASN, 5-8 November 1999.
  12. Efendiev, R., Bertorello, A., Pedemonte, C. Dopamine inhibition and PMA activation of proximal tubule Na,K-ATPase activity are mediated by PKC-zeta and PKC-beta, respectively. 32nd Annual meeting of ASN, 5-8 November 1999.
  13. Efendiev, R., Bertorello, A., Pressley, T., Pedemonte, C. Stimulation of Na,K-ATPase by PMA is mediated by recruitment of transport molecules to the plasma membrane. 32nd Annual meeting of ASN, 5-8 November 1999.
  14. Efendiev, R., Bertorello, A., Pedemonte, C. Dopamine inhibition and PMA activation of proximal tubule Na,K-ATPase involve different PKC isoforms. Annual meeting of ASCB, 11-15 December 1999.

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