B. Publications
1. Ruan, K.-H., Imagawa, M., Hashida, S., and Ishikawa,
E.: An improved method for the conjugation of Fab' to ?-D-galactosidase
from Escherichia coli. Anal. Lett. 17:539-554, 1984.
2. Ruan, K.-H., Hashida, S., and Ishikawa, E.: A
micro-scale preparation of affinity-purified Fab'-enzyme conjugates
with high purity for enzyme immunoassay. Anal. Lett. 17(B 1 8):2075-2090,
1984.
3. Hashida, S., Imagawa, M., Inoue, S., Ruan, K.-H., and
Ishikawa, E.: More useful maleimide compounds for the conjugation
of Fab'-to horseradish peroxidase through thiol groups in the
hinge. J. Appl. Biochem. 6:56-63, 1984.
4. Inoue, S., Imagawa, M., Hashida, S., Ruan K-H, and Ishikawa,
E.: A small scale preparation of affinity-purified rabbit Fab’-horseradish
conjugate for enzyme immunoassay. Anal. Lett. 17 (B3):229-242,1984.
5. Ruan, K.-H., Hashida, S., and Yoshitake, S.,
Ishikawa, E., Walkisaka, O., Yamamoto, Y., Ichioka, T., and Nakajima,
K.: A micro-scale affinity-purification of Fab'-horseradish peroxidase
conjugates and its use for sandwich enzyme immunoassay of insulin
in human serum. Clin. Chim. Acta 147:167-172, 1985.
6. Hashida, S., Ruan, K.-H., and Ishikawa, E.: A micro-scale
affinity purification of Fab'enzyme conjugates: VTV International
Conference on Labeled Antibodies (Tokyo) November, 1985.
7. Ruan, K.-H., Hashida, S., Yoshitake, S., Ishikawa, E.,
Wakisaka, O., Yamamoto, Y., Ichioka, T., and Nakajima, K.: A more
sensitive and less time-consuming sandwich enzyme immunoassay
for insulin in human serum with less serum interference. Ann.
Clin. Biochem. 23:54-58, 1986.
8. Kohno, T., Ruan, K.-H., and Ishikawa, E.: A highly sensitive
enzyme immunoassay of anti-insulin antibodies in guinea pig serum.
Anal. Lett. 19. 1083-1095, 1986.
9. Ruan, K.-H., Hashida, S., Tanaka, K., and Ishikawa,
E.: A small-scale sandwich enzyme immunoassay for macromolecular
antigens using beta-D-galactosidase from Escherichia coli and
horseradish peroxidase as labels. Anal. Lett. 20 (4):587-601,
1987.
10. Ishikawa, E., Hashida, S., Kohono, T., Tanaka, K., Ruan,
K.-H., and Ishoue, S.: Enzyme labeling of antibodies and enzyme
immunoassay. in: Cellular, Molecular and Genetic Approaches to
Immunodiagnosis and Immunotherapy. (Kano, K., Mori, S., Sugisaki,
T., and Rorisu, M., eds). Pp.116-128, 1987.
11. Ruan, K.-H., Ke, D.-R., Hang, S.-W., Ni, D.-R., Pan
S.-Z., Yao, R.-H, Lin, H.-X., and Xie, Z.-C.: Determination of
insulin in human saliva using a more sensitive sandwich enzyme
immunoassay. Anal. Lett. 21:381-394,1988.
12. Ruan, K.-H. and Ke, D.-R..: Small-scale sandwich enzyme
immunoassay for human chorionic gonadotropin using a new micro-transferable
plastic bead. Ann. Clin. Biochem. 25:645-649, 1988.
13. Atassi, M. Zhouhair, Ruan, K.-H., Spurlino, J., and
Quiocho, F.: Determination of the region to-region contacts and
the disposition of ?-bungarotoxin within the toxin-binding site
on human acetylcholine receptor. J. Prot. Chem. 9:349-3509, 1990.
14. Ruan, K.-H., Spurlino, J., Quiocho, F.A., and Atassi,
M.Z.: Acetylcholine receptor-bungarotoxin interactions: Determination
of the region-to-region contacts by peptide-peptide interactions
and molecular modeling. Proc. Natl. Acad. Sci. 87:6156-6160, 1990.
15. Ruan, K.-H. and Atassi, M.Z.: Full profile of the short
neurotoxin-binding regions on the extracellular part of the a-chain
of the acetylcholine receptor. Biochem. J. 274:849-854, 1991.
16. Ashizawa, T., Ruan, K.-H., Jinnai, K., and Atassi,
M.Z.: Profile of the regions on alpha-chain of human acetylcholine
receptor recognized by autoantibodies in Myasthenia Gravis. Mol.
Immunol. 29:1507-1514,1992.
17. Atassi, M.Z., Ruan, K.-H., Jinnai, K., Oshima, M.,
and Ashizawa, T.: Epitope-specific suppression of antibody response
in experimental autoimmune myasthenia gravis by a monoethoxypolyethylene
glycol conjugate of a myasthenogenic synthetic peptide. Proc.
Natl. Acad. Sci. USA 89:5852-5856, 1992.
18. Ohashi, K., Ruan, K.-H., Kulmacz, R.J., Wu, K.K. and
Wang, L.-H.: Primary structure of human thromboxane synthase determined
from the cDNA sequence. J. Biol. Chem. 267, 789-793, 1992.
19. Yio, Y.-Y., Jian, J., Yin, F.-Z., and Ruan, K.-H.:
highly sensitive sandwich enzyme immunoassay for alpha-fetoprotein
in human saliva. Ann. Clin. Biochem. 29, 519-522, 1992.
20. Ruan, K.-H., Wang, L.-H., Wu, K.K., and Kulmacz, R.J.:
NH2-terminal topology of thromboxane synthase in the endoplasmic
reticulum. J. Biol. Chem. 268:19483-19490, 1993.
21. Ruan, K.-H., Kulmacz, R.J., Wilson, A., and Wu, K.K.:
Highly sensitive fluorimetric enzyme immunoassay for prostaglandin
H synthase solubilized from cultured cells. J. Immunol. Methods.
162:23-30, 1993.
22. Xu, X.-M., Ohashi, K., Sanduja, S.K., Ruan, K.-H.,
Wang, L.-H., and Wu, K.K.: Enhanced prostacyclin synthesis in
endothelial cells by retrovirus-mediated transfer of prostaglandin
H synthase cDNA. J. Clin. Invest. 91:1843-1849, 1993.
23. Ruan, K.-H., Milfeld, K., Kulmacz, R.J., and Wu, K.K.:
Construction of a three-dimensional model of human thromboxane
synthase: implication for substrate-binding pocket. Protein Engin.
7:1345-1351, 1994.
24. Ruan, K.-H., Ping, L., Kulmacz, R.J., and Wu, K.K.:
Characterization of the structure and membrane interaction of
NH2-terminal domain of thromboxane A2 synthase. J. Biol. Chem.
269:20938-20942, 1994.
25. Ren, Y., Walker, C., Loose-Mitchell, D.S., Deng, J., Ruan,
K.-H., and Kulmacz, R.J.: Topology of prostaglandin H synthase-1
in the endoplasmic reticulum membrane. Arch. Biochem. Biophys.
323, 205-214, 1995.
26. Guo, Q., Wang, L.-H., Ruan, K.-H., and Kulmacz, R.J.:
Role of Val509 in time-dependent inhibition of human prostaglandin
H synthase-2 cyclooxygenase activity by isoform-selective agents.
J Biol. Chem. 271. 19134-19139, 1996.
27. Wang, L.-H., Matijevic-Aleksic, N., Hsu, P.-Y., Ruan, K.-H.,
Wu, K.K. and Kulmacz, R.J.: Identification of thromboxane A2 synthase
active site residues by molecular modeling-guided site-directed
mutagenesis. J. Biol. Chem. 271, 19970-19975, 1996.
28. Shyue, S., Ruan, K.-H., Wang, L.-H., and Wu, K.K.:
Prostacyclin synthase active sites: Identification by molecular
modeling-guided site-directed mutagenesis. J. Biol. Chem. 272,
3657-3662, 1997.
29. Ruan, K.-H., Li, D., Ji, J., Lin, Y. -Z., and Gao,
X.: Structural characterization and topology of the second potential
membrane anchor region in the thromboxane A2 synthase amino terminal
domain. Biochemistry, 37, 822-830, 1998.
30. Lin, Y.-Z., Wu, K.K. and Ruan, K.-H.: Characterization
of secondary structure and membrane interaction of the putative
membrane anchor domains of prostaglandin I2 synthase and cytochrome
P450 2C1. Arch. Biochem. Biophys. 352, 78-84, 1998.
31. Lin, Y-Z., Deng, H. and Ruan, K.-H.: Topology of catalytic
portion of prostaglandin I2 synthase: Identification by molecular
modeling-guided site-specific antibodies. Arch. Biochem. and Biophys.
379, 188-197, 2000.
32. So, S-P., Li, D. and Ruan, K.-H.: Identification of
the substrate-interaction site in the N-terminal membrane anchor
segment of thromboxane A2 synthase by determination of its substrate
analog conformation changes using high resolution NMR technique.
J. Biol. Chem. 275, 40679-40685, 2000.
33. Ruan, K.-H., So, S-P, Wu, J., Huang, A. and Kung J.
Solution structure of second extracellular loop of human thromboxane
A2 receptor. Biochemistry, 40, 275-280, 2001.
34. Deng, W.G., Ruan, K.-H, Du, M., Sauders, M., and Wu, K.K.
Aspirin and salicylate bind to immunoglobulin heavy-chain binding
protein (BiP) and inhibit its ATPase activity in human fibroblasts.
FASEB J. 15, Nov. 2001.
35. Arbab, F., Goldsby, J. S., Matijevic-Aleksic, N., Huang, G.,
Ruan, K.-H., and Huang, J-C. Prostacyclin is an autocrine
regulator in the contraction of oviductal smooth muscle, Hum Reprod.
17, 3053-3059, 2002.
36. Deng, H., Huang A., So, S.-P., Lin, Y.-Z. and Ruan, K.-H.
Substrate access channel topology in membrane-bound prostacyclin
synthase. Biochem. J., 362, 545-551, 2002.
37. Ruan, K.-H, So, S.-P., Zheng, W., Wu, J.,
Li, D, Kung, J., Li, D., Kung, J. Solution Structure and topology
of the NH2-Terminal membrane anchor domain of a microsomal P450:
Prostaglandin I2 Synthase. Biochem. J. 368, 721-728, 2002.
38. So, S.-P., Wu, J., Huang, G., Huang, A., Li, D., Ruan,
K.-H. Identification of Residues Important for Ligand Binding
of Thromboxane A2 Receptor in the Second Extracellular Loop Using
the NMR Experiment-guided Mutagenesis Approach. J. Biol. Chem.
278, 10922-10927, 2003.
39. Wu, J., So, S.-P., Ruan, K.-H. Determination of the
membrane contact residues and solution structure of the helix
F/G loop of prostaglandin I2 synthase. Arch. Biochem. Biophys.
411, 27-35, 2003.
40. Deng, H., Wu, J., So, S.-P., Ruan, K.-H. Identification
of the residues in the helix F/G loop important to catalytic function
of membrane-bound prostacyclin synthase. Biochemistry, 42, 5609-5617,
2003.
41. Wu, J., So, S.-P., and Ruan, K.-H. Solution structure
of the third extracellular loop of human thromboxane A2 receptor.
Arch. Biochem. Biophys. 414, 287-293, 2003.
42. Ruan, K.-H., Wu, J., So, S.-P., and Jenkins, L. A.
Evidence of the residues involved in ligand recognition in the
second extracellular loop of the prostacyclin receptor characterized
by high resolution 2D NMR techniques. Arch. Biochem. Biophys.
418, 25-33, 2003.
43. Geng, L., Wu, J., So, S.-P., and Ruan, K.-H. Structural
and functional characterization of the first intracellular loop
of thromboxane A2 receptor. Arch. Biochem. Biophys, 423, 253-265,
2004.
44. Dogne JM, de Leval X, Hanson J, Frederich M, Lambermont B,
Ghuysen A, Casini A, Masereel B, Ruan KH, Pirotte
B, Kolh P. New developments on thromboxane and prostacyclin modulators
part I: thromboxane modulators. Curr Med Chem. 11, 1223-1241,
2004.
45. Ruan, K.-H. Advances in biosynthesis of thromboxane
A2 and prostacyclin in endoplasmic reticulum membrane via cyclooxygenase
pathway. Mini Rev. Med. Chem., 4, 639-647, 2004.
46. Ruan, K.-H. High resolution NMR spectroscopy-guided
mutagenesis for characterization of membrane-bound proteins: experimental
designs and applications. Spectroscopy, 18, 13-29, 2004.
47. Ruan, K.H., Wu, J., So, S., Jenkins, L. and Ruan, CH. NMR
structure of the thromboxane A2 receptor ligand recognition pocket.
Eur. J. Biochem. 271, 1–11, 2004.
48. Ruan, K.-H, Deng, H, Wu, J., So, SP. The N-terminal membrane
anchor domain of the membrane-bound prostacyclin synthase involved
in the substrate presentation of the coupling reaction with cyclooxygenase.
Arch. Biochem. Biophys, 435, 372-381, 2005.
49. Ruan, K.-H. Thrombogenesis and anti-thrombogenesis
in atherosclerosis mediated by prostanoids through the cyclooxygenase
pathway. Chapter V, in: current Topics in Atherosclerosis Research.
Editor Y.J. Geng, Nova Science Publishers Inc. New York, 2005.
50. Ruan, K.-H., Wu, J, Wang LH. Solution structure of
a common substrate mimetic of cyclooxygenase-downstream synthases
bound to an engineered thromboxane A2 synthase using a high-resolution
NMR technique. Arch Biochem Biophys 444, 165-173, 2005.
51. Ruan, CH, Wu, J., Ruan K-H. A strategy using
NMR peptide structures of thrombxane A2 recetor as templates to
construct ligand-recognition pocket of prostacyclin receptor.
BMC Biochem. 6:23, 2005.
52. Zhang, L., Huang, G., Wu, J Ruan K-H. A profile
of the residues in the first intracellular loop critical for Gs-mediated
signalling of human prostacyclin receptor characterized by an
integrative approach of MR-experiment and mutagenesis. Biochemistry
44:11389-11401, 2005.
53. Dogné, J., Hanson, J., Leval, X., Pratico, D., Pace-Asciak,
C., Pirotte, B and Ruan, K.-H. From the design to the clinical
application of thromboxane modulators. Curr Pharm Design, 12,
903-923, 2006.
54. Ruan, K.-H. and Dogné, J. Implications of the
molecular basis of prostacyclin biosynthesis and signaling in
pharmaceutical designs. Curr Pharm Design, 12, 925-941, 2006.
55. Huang, JC, Ruan, CH, Tang, K and Ruan, KH.
Prunella stica inhibits the proliferation but not the prostaglandin
production of Ishikawa cells. Life Sci. 79, 436-441, 2006.
56. Dogne JM, Ruan KH. Latest developments in
pharmaceutical design of arachidonic acid metabolites: prostaglandsin,
thromboxanes, hepoxillins and isoprostanes. Curr Pharm Des. 12,
893, 2006.
57. Zhang, L., Wu, J., Ruan KH. Solution structure
of the first intracellular loop of prostacyclin receptor and implication
of its interaction with the C-terminal segment of G alpha S protein.
Biochemistry 45, 1734-1744, 2006.
58. Zhang L, Bastepe M, Juppner H, Ruan KH. Characterization
of the molecular mechanisms of the coupling between intracellular
loops of prostacyclin receptor with the C-terminal domain of the
Galphas protein in human coronary artery smooth muscle cells.
Arch Biochem Biophys. Jul 25, 2006.
59. Ruan KH, Deng H, So SP. Engineering of a
protein with cyclooxygenase and prostacyclin synthase activities
that converts arachidonic acid to prostacyclin. Biochemistry.
2006 45(47):14003-14011.
60. Ruan KH, Wu J, Cervantes V. Characterization
of the Substrate Mimic Bound to Engineered Prostacyclin Synthase
in Solution Using High-Resolution NMR Spectroscopy and Mutagenesis:
Implication of the Molecular Mechanism in Biosynthesis of Prostacyclin.
Biochemistry. 2008 Biochemistry. 47(2):680-688.
61. Wu J, Feng M, Ruan KH. Assembling NMR structures
for the intracellular loops of the human thromboxane A2 receptor:
Implication of the G protein-coupling pocket. Arch Biochem Biophys.
2008;470(1):73-82.
62. Ni F, So SP, Cervantes V,
Ruan KH. A profile of the residues in the second
extracellular loop that are critical for ligand recognition of
human prostacyclin receptor. FEBS J. 2008;275(1):128-137.
